반응 #833537

ord-b2f7a63f9a06431dac68f45da5d4ecf1

반응 방정식

NC(CO)(CO)CO.O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate Tris
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
phosphate buffered saline
→
CC(C)=CCC/C(C)=C/CC/C(C)=C/CC/C=C(\C)CC/C=C(\C)CCC=C(C)C
MF59

반응물

—
MF59-0
NC(CO)(CO)CO.O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate Tris
—
2
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
phosphate buffered saline
—
MF59-0

시약

없음

반응 조건

상세 조건
See reaction.notes.procedure_details.

후처리

  1. 1
    기타to yield a total of 0.7 ml
  2. 2
    workup.ADDITIONThe composition was gently mixed
  3. 3
    기타produced in Example 1
  4. 4
    기타to yield a total of 3 ml
  5. 5
    workup.ADDITIONThe composition was gently mixed

실험 절차

10 Baboons were divided into two groups (five baboons per group) and administered the formulations specified in Table 1. For Group 1, equal parts of the adjuvant MF59-0, and p24gag/sf2 (in a citrate/Tris buffer) were combined to yield a total of 0.7 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected intramuscularly (IM) in the thigh muscle. For Group 2, 1.5 ml of 2× phosphate buffered saline (PBS) was added to 46.8 mg of the PLG-entrapped p24gag/sf2 formulation produced in Example 1. The material was vortexed for about 30 seconds until all beads were in suspension. 1.5 ml of MF59-0 was added to the resuspended beads to yield a total of 3 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected IM in the thigh muscle.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US07393630B2uspto-grants-2008_07