반응 #828720

반응 방정식

sodium hydroxide

glycine

→

glucose

반응 조건

후처리

1. 1
   workup.WAITThese cultures were cultivated for 18 hours under the above cultural environment
2. 2
   기타were collected by centrifugation (3500 rpm, 10 min.)
3. 3
   workup.ADDITIONcontaining the following components

실험 절차

The BIBB 10 medium was adjusted to pH 7.0 with 10% sodium hydroxide solution before sterilization. The culture medium was shaken on a rotary shaker at 260 rpm for 24 hours at 28° C., then 3 ml of same were inoculated into 100 ml of fresh BIBB 10 medium supplemented with 0.5% of glycine before inoculation. These cultures were cultivated for 18 hours under the above cultural environment, and after checking the cells were collected by centrifugation (3500 rpm, 10 min.). The mycelium derived from 100 ml of the culture broth was suspended in 4 ml of PH mixture (pH 7.0) containing the following components:

출처