반응 #82861

ord-4b47fa3023224dd4ad90ab028285444d

반응 방정식

$CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

chloroform

→

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC.CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA DOPC

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

반응물

$CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

chloroform

시약

없음

반응 조건

상세 조건

See reaction.notes.procedure_details.

후처리

1
기타removing the solvent
2
기타by drying under a stream of nitrogen gas
3
기타was placed under vacuum overnight
4
기타to remove traces of solvent
5
기타sonicated in a bath type sonicator for 60 minutes until clear,

실험 절차

DOTMA/DOPC SUV's were prepared by dissolving 40 mg DOTMA and 60 mg DOPC together in chloroform and removing the solvent by drying under a stream of nitrogen gas. This dried film was placed under vacuum overnight to remove traces of solvent. The film was then suspended in 2 ml water with vigorous shaking and sonicated in a bath type sonicator for 60 minutes until clear, producing DOTHA/DOPC vesicles at a concentration of 50 mg/ml.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US05622712uspto-grants-1997_04