반응 #82860

ord-1825b608ebc244d3b221ee45bb66642a

반응 방정식

$CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

→

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC.CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA DOPC

반응물

$CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

시약

없음

용매

chloroform

반응 조건

상세 조건

See reaction.notes.procedure_details.

후처리

1
기타DOTMA/DOPC vesicles were prepared
2
기타removing the solvent
3
기타by drying under a stream of nitrogen gas
4
기타was placed under vacuum overnight
5
기타to remove traces of solvent
6
기타sonicated in a bath type sonicator for 30 minutes

실험 절차

DOTMA/DOPC vesicles were prepared by dissolving 10 mg DOTMA and 10 mg DOPC in chloroform and removing the solvent by drying under a stream of nitrogen gas. This dried film was placed under vacuum overnight to remove traces of solvent. The film was then suspended in 1 ml water with vigorous shaking and sonicated in a bath type sonicator for 30 minutes until clear producing DOTMA/DOPC SUV's at a concentration of 20 mg/ml total lipid.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US05622712uspto-grants-1997_04