반응 #76833

반응 방정식

1,3-dicyclohexylcarbodiimide

(R)-9-[4-Hydroxy-2-(stearoyloxymethyl)butyl]guanine

N-BOC-L-isoleucine

→

(R)-9-[4-(N-BOC-L-isoleucyloxy)-2-(stearoyloxymethyl)butyl]guanine

용매

—

solvent

DMF

반응 조건

후처리

1. 1
   기타After reaction for 18 hours
2. 2
   여과the reaction mixture was filtered through Celite
3. 3
   기타The product was isolated by silica gel column chromatography
4. 4
   세척eluting at 5% MeOH in CH2Cl2

실험 절차

(R)-9-[4-Hydroxy-2-(stearoyloxymethyl)butyl]guanine (135 mg, 0.26 mmole) and N-BOC-L-isoleucine (180 mg, 0.78 mmole) were coevaporated with dry DMF twice and dissolved in the same solvent (3.5 ml). To the solution was added 1,3-dicyclohexylcarbodiimide (160 mg, 0.78 mmole) and 4-dimethylaminopyridine (4.8 mg, 0.039 mmole). After reaction for 18 hours, the reaction mixture was filtered through Celite and worked up in a conventional manner. The product was isolated by silica gel column chromatography, eluting at 5% MeOH in CH2Cl2. (Yield 160 mg)

출처