반응 #68769

ord-28b9f6fe397143c3afbbfc2187eacc8c

반응 방정식

COc1ccc(-c2oc3c(CC=C(C)C)c(O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)cc(O)c3c(=O)c2O[C@@H]2O[C@@H](C)[C@H](O)[C@@H](O)[C@H]2O)cc1
icariin
O=P([O-])(O)O.O=P([O-])([O-])O.[Na+].[Na+].[Na+]
sodium dihydrogen phosphate disodium hydrogen phosphate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
→
COc1ccc(-c2oc3c(CC=C(C)C)c(O)cc(O)c3c(=O)c2O[C@@H]2O[C@H](C)[C@@H](O)[C@H](O)[C@@H]2O)cc1
icariside II

반응물

COc1ccc(-c2oc3c(CC=C(C)C)c(O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)cc(O)c3c(=O)c2O[C@@H]2O[C@@H](C)[C@H](O)[C@@H](O)[C@H]2O)cc1
icariin
O=P([O-])(O)O.O=P([O-])([O-])O.[Na+].[Na+].[Na+]
sodium dihydrogen phosphate disodium hydrogen phosphate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose

시약

없음

용매

—
β

반응 조건

온도
40°CELSIUS
상세 조건
See reaction.notes.procedure_details.

후처리

  1. 1
    기타to remove glucoside
  2. 2
    온도Then stop heating
  3. 3
    추출repeatedly extract with ethyl acetate
  4. 4
    기타dried
  5. 5
    추출Then repeatedly extract with methanol

실험 절차

Fifty grams icariin were added into sodium dihydrogen phosphate-disodium hydrogen phosphate buffer (pH5.5) and 100 ml β glucose. 100 ml of β glucosidase was added and the solution was heated to 40° C. with stirring for 24 hours in order to remove glucoside. Then stop heating and repeatedly extract with ethyl acetate. The ethyl acetate layer was pooled and dried. Then repeatedly extract with methanol. The HPLC analysis confirmed that the content of pure icariside II 50.1%. Thus extract comprising icariside II is obtained.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US08530433B2uspto-grants-2013_09