반응 #65116

반응 방정식

NaOH

N-benzoyl-L-aspartic acid

L-phenylalanine methyl ester

acetonitrile

→

N-benzoyl-L-aspartyl-L-phenylalanine methyl ester

용매

water

반응 조건

후처리

1. 1
   여과filtered immobilized enzyme
2. 2
   기타prepared above
3. 3
   기타After completion of the reaction
4. 4
   기타the immobilized enzyme was separated by filtration
5. 5
   기타Resulting slurry
6. 6
   여과was filtered
7. 7
   세척washed with water

실험 절차

1.19 g (5.0 mmol) of N-benzoyl-L-aspartic acid and 1.79 g (10.0 mmol) of L-phenylalanine methyl ester were added to a flask which contained 50 ml of 50% acetonitrile in water containing 5 mM CaCl2. The pH of the solution was adjusted to 6.0 with 10M NaOH. The solution was admixed with 4 g of suction filtered immobilized enzyme prepared above. Thereafter, the reaction were carried out with stirring for 4 hours at 40° C. After completion of the reaction, the immobilized enzyme was separated by filtration. The pH of the filtrate was then adjusted to 3.7 by 10 molar hydrochloric acid. Resulting slurry was filtered and washed with water to give N-benzoyl-L-aspartyl-L-phenylalanine methyl ester.

출처