반응 #60616

반응 방정식

Boc-Leu-OSu

Glu-OtBu

NMM

→

Boc-Leu-Glu-OtBu

용매

DMF

반응 조건

후처리

1. 1
   기타the solvent was removed
2. 2
   세척washed 3× with pH 3 water (100 ml)
3. 3
   건조Organic layer was dried with MgSO4
4. 4
   여과filtered
5. 5
   기타Solvent was removed
6. 6
   기타solid was dried over vacuum
7. 7
   기타Solid was collected as a white powder (8.78 g, quant. yield, ˜80% purity TLC)

실험 절차

To a solution of Boc-Leu-OSu and Glu-OtBu in DMF was added NMM and DMAP. The solution was stirred at ambient temperatures for 18 hours then the solvent was removed. Crude product was taken up in 100 ml CHCl3, washed 3× with pH 3 water (100 ml), once with water (100 ml) and once with brine (100 ml). Organic layer was dried with MgSO4 and filtered. Solvent was removed and solid was dried over vacuum. Solid was collected as a white powder (8.78 g, quant. yield, ˜80% purity TLC). Product was used directly without further characterization.

출처