반응 #5966

반응 방정식

9-(3-hydroxymethyl-4-monomethoxytrityloxybut-1-yl)-2-monomethoxytritylaminopurine

butyric anhydride

N,N-dimethylformamide

Methanol

→

2-amino-9-(4-butyryloxy-3-hydroxymethylbut-1-yl)purine

반응 조건

후처리

1. 1
   기타the solvent was removed
2. 2
   workup.STIRRINGthe solution was stirred at 70° for 30 minutes
3. 3
   workup.ADDITIONWater (2 ml) was added
4. 4
   추출the solution was extracted with hexane (2×10 ml)
5. 5
   기타the solvent was removed
6. 6
   기타The residue was partitioned between saturated aqueous sodium bicarbonate and chloroform
7. 7
   건조the organic layer was dried (magnesium sulphate)
8. 8
   기타the solvent removed
9. 9
   기타The residue was purified by column chromatography on silica gel eluting with chloroform-methanol (16:1

실험 절차

To a solution of 9-(3-hydroxymethyl-4-monomethoxytrityloxybut-1-yl)-2-monomethoxytritylaminopurine (0.70 g, 0.9 mmol) and 4-dimethylaminopyridine (10 mg) in N,N-dimethylformamide (5 ml) was added butyric anhydride (0.29 ml, 1.8 mmol) and the solution was stirred for 15 minutes. Methanol (1 ml) was added and the solvent was removed. The residue was taken up in 80% acetic acid (9 ml) and the solution was stirred at 70° for 30 minutes. Water (2 ml) was added and the solution was extracted with hexane (2×10 ml). The aqueous layer was retained and the solvent was removed. The residue was partitioned between saturated aqueous sodium bicarbonate and chloroform and the organic layer was dried (magnesium sulphate) and the solvent removed. The residue was purified by column chromatography on silica gel eluting with chloroform-methanol (16:1 to afford 2-amino-9-(4-butyryloxy-3-hydroxymethylbut-1-yl)purine which was obtained as a white crystalline solid after trituration with methanol (188 mg, 68%), m.p. 125°-127°; λmax (MeOH) 222 (27,600), 243 (4,830), and 308 (6,950)nm; νmax (KBr) 3190, 1730, 1640, 1620, and 1580 cm-1 ; δH [(CD3)2SO]0.85 (3H, t, J 7.4 Hz, CH3), 1.50 (2H, sextet, J 7.3 Hz, CH2CH2CH3), 1.68 (1H, m, 3'-H), 1.82 (2H, m, 2'-H), 2.23 (2H, t, J 7.4 Hz, CH2CH2CH3), 3.42 (2H, t, J 5.2 Hz, D2O exchange gives d, CH2OH), 3.95-4.1 (2H, ABX, JAB 11.0 Hz, JAX =JBX 5.8 Hz, CH2OOC), 4.12 (2H, t, J 7.3 Hz, 1'-H), 4.62 (1H, t, J 4.9 Hz, D2O exchangeable, OH), 6.44 (2H, s, D2O exchangeable, 2-NH2), 8.06 (1H, s, 8-H), and 8.56 (1H, s, 6-H); (Found: C, 54.41; H, 6.91; N, 22.70%. C14H21N5O3 requires C, 54.71; H, 6.89; N, 22.79%).

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