반응 #58616

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Me3Al

(S)-3-amino-azepane-1-carboxylic acid tert-butyl ester

Rochelle's salt

methyl 5-(4-methoxyphenyl)-2-({[(trichloroacetyl)amino]carbonyl}amino)thiophene-3-carboxylate

→

title compound

수율 62.0%

tert-Butyl(3S)-3-({[2-[(aminocarbonyl)amino]-5-(4-methoxyphenyl)-3-thienyl]carbonyl}amino)azepane-1-carboxylate

수율 62.0%

용매

THF

EtOAc

H2O

THF

THF

반응 조건

후처리

1. 1
   workup.STIRRINGThe resulting deep yellow/orange solution was stirred overnight at room temperature
2. 2
   온도The reaction mixture was cooled with ice
3. 3
   기타to quench
4. 4
   기타the reaction
5. 5
   온도The resulting biphasic solution was warmed to room temperature
6. 6
   workup.STIRRINGstirred for an additional 1 h
7. 7
   추출the aqueous layer was extracted with EtOAc (3×)
8. 8
   세척the combined organic extracts were washed with H2O, brine
9. 9
   건조dried (Na2SO4)
10. 10
    기타Evaporation
11. 11
    기타gave a pale orange solid
12. 12
    기타Purification by ISCO MPLC (SiO2, 60-80% EtOAc/hexanes)

실험 절차

To a solution of methyl 5-(4-methoxyphenyl)-2-({[(trichloroacetyl)amino]carbonyl}amino)thiophene-3-carboxylate (1.36 g, 3 mmol) in anhydrous THF (20 mL) was added a solution of [Me2Al-3-Boc-(S)-3-aminohomopiperidine] in THF (preformed by the careful addition of Me3Al (2.0 M in hexanes, 3.0 mL, 6.0 mmol) to a solution of (S)-3-amino-azepane-1-carboxylic acid tert-butyl ester in 10 nL of THF at −78° C. followed by warming to room temperature under nitrogen and stirring for an additional 15 min.). The resulting deep yellow/orange solution was stirred overnight at room temperature. The reaction mixture was cooled with ice and a 10% aqueous solution of Rochelle's salt was added slowly to quench the reaction. The resulting biphasic solution was warmed to room temperature and stirred for an additional 1 h. The mixture was diluted with EtOAc and H2O, the aqueous layer was extracted with EtOAc (3×) and the combined organic extracts were washed with H2O, brine and dried (Na2SO4). Evaporation gave a pale orange solid. Purification by ISCO MPLC (SiO2, 60-80% EtOAc/hexanes) gave 0.9 g (62%) of the title compound as a white solid. 1H NMR (d6-DMSO, δ 11.0, s, 1H, δ 7.95, d, 0.5H, δ 7.81, d, 0.5H, δ 7.65, s, 0.5H; δ 7.56, s, 0.5H; δ 7.46, d, 2H; δ 6.97, d, 2H; δ 6.96, br s, 2H; δ 4.19, m, 0.5H; δ 4.11, m, 0.5H; δ 3.77, m, 3H; δ 3.65, m, 1H; δ 3.48, m, 1H; δ 3.20, m, 2H; δ 1.75, m, 3H; δ 1.58, m, 2H; δ 1.42, s, 4.5H; δ 1.39, m, 1H; δ 1.36, s, 4.5H), LC/MS (APCI, ES, M+H=489).

출처