반응 #58480

반응 방정식

X-100

dihydroorotate

2,6-dichloroindophenol

dihydroorotate

KCl

→

2,5-dihydro furan 3,4-dicarboxylic acid

용매

TrisHCl

반응 조건

후처리

1. 1
   기타at 30° C.
2. 2
   workup.ADDITIONThe components were mixed
3. 3
   기타The course of reaction
4. 4
   기타in absorption at 600 nm for 2 min.

실험 절차

The standard assay mixture contained 50 μM decyclo ubichinone, 100 μM dihydroorotate, 60 μM 2,6-dichloroindophenol, as well as 20 mU DHODH. The volume activity of the recombinant enzyme used was 30 U/ml. Measurements were conducted in 50 mM TrisHCl (150 mM KCl, 0,1% Triton X-100, pH. 8,0) at 30° C. in a final volume of 1 ml. The components were mixed, and the reaction was started by adding dihydroorotate. The course of reaction was followed by spectrophotometrically measuring the decrease in absorption at 600 nm for 2 min.

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