반응 #554589

반응 방정식

sodium hydroxide

phosphoric acid

phytic acid

→

phytate

용매

water

반응 조건

후처리

1. 1
   추출oil therein was extracted
2. 2
   기타separated
3. 3
   기타removed
4. 4
   기타to obtain low
5. 5
   추출Next, it was extracted
6. 6
   기타to separate bean curd refuse and insolubles
7. 7
   기타to obtain
8. 8
   기타to obtain
9. 9
   기타an insoluble fraction (acid precipitated curd)
10. 10
    workup.ADDITIONWater was added so that the acid
11. 11
    기타precipitated curd
12. 12
    기타to obtain an acid
13. 13
    기타precipitated curd slurry
14. 14
    온도was heated to 40° C
15. 15
    workup.ADDITIONTo this solution (phytic acid content 1.96% by mass; solid content, TCA solubilization ratio 4.6%) was added phytase (manufactured by NOVO, Inc.) of equivalent to 8 units per solid content
16. 16
    workup.WAITto act for 30 minutes
17. 17
    기타After the reaction
18. 18
    온도heat treatment
19. 19
    workup.WAITwas performed at 120° C. for 15 seconds
20. 20
    기타This was then spray dried

실험 절차

Soybeans were pressurized, and then oil therein was extracted, separated, and removed using n-hexane as an extraction solvent to obtain low denatured defatted soybeans. To 5 kg of the obtained low denatured defatted soybeans (nitrogen solubility index (NSI) 91) was added 35 kg of water, and the pH was adjusted to 7 using diluted sodium hydroxide solution. Next, it was extracted while stirring for one hour at room temperature, and then centrifuged at 4000 G to separate bean curd refuse and insolubles, to obtain defatted soybean milk. The pH of this defatted soybean milk was adjusted to 4.5 using phosphoric acid, and then centrifuged using a continuous centrifugal separator (decanter) at 2000 G, to obtain an insoluble fraction (acid precipitated curd) and a soluble fraction (whey). Water was added so that the acid precipitated curd became 10% by mass of solid content, to obtain an acid precipitated curd slurry. After the pH of this was adjusted to 4.0 using phosphoric acid, it was heated to 40° C. To this solution (phytic acid content 1.96% by mass; solid content, TCA solubilization ratio 4.6%) was added phytase (manufactured by NOVO, Inc.) of equivalent to 8 units per solid content, allowing the enzymes to act for 30 minutes. After the reaction, the pH of this enzyme-applied product (phytic acid content 0.04% by mass; solid content, TCA solubilization ratio substantially unchanged) was adjusted to 3.5, and heat treatment was performed at 120° C. for 15 seconds, using a continuous direct heat treatment sterilizer. This was then spray dried, to obtain 1.5 kg of low phytate soybean protein powder. The dissolution ratio of this protein (pH 3.5) was 95%.

출처