반응 #465931

반응 방정식

p-phenylenediamine

DI water

→

1-naphthol

용매

CH3COONa

반응 조건

후처리

1. 1
   workup.ADDITION100 ml “A” was added to one beaker and 50 ml “A” and 50 ml “B”
2. 2
   기타to form 100 ml in a second beaker
3. 3
   기타The LOM beakers were sealed
4. 4
   workup.ADDITIONThe spent liquor was poured off
5. 5
   세척the swatches were rinsed in cold tap water for about 15 minutes
6. 6
   기타The swatches were dried at room temperature CIELAB values

실험 절차

A 0.5 mg/ml solution of a first compound (p-phenylenediamine, “A”) and a 0.5 mg/ml solution of a second compound (1-naphthol, “B”) was prepared by dissolving the compound in the appropriate amount of 0.1 M CH3COONa, pH 5.5, buffer. A total volume of 100 ml was used in each LOM beaker. 100 ml “A” was added to one beaker and 50 ml “A” and 50 ml “B” were combined to form 100 ml in a second beaker. Swatches of the materials listed above were wetted in DI water and soaked in the precursor solutions. A Polyporus pinsitus laccase (“PpL”) with an activity of 70 LACU/ml (100 LACU/mg) was added to each beaker at a concentration of 12.5 mg/l. The LOM beakers were sealed and mounted in the LOM. After 1 hour at 42 RPM and 30° C., the LOM was stopped. The spent liquor was poured off and the swatches were rinsed in cold tap water for about 15 minutes. The swatches were dried at room temperature CIELAB values were measured for all of the swatches using the Macbeth ColorEye 7000. The results are given in Tables 23 and 24.

출처