반응 #450893

ord-b4112dba39db4f869c590e5c996c75c1

반응 방정식

CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
SDS
NC(CO)(CO)CO
Tris
OCC(O)CO
glycerol
CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
SDS
CC(O)S
mercaptoethanol
→
CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
IPTG

반응물

CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
SDS
NC(CO)(CO)CO
Tris
OCC(O)CO
glycerol
CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
SDS
CC(O)S
mercaptoethanol

시약

없음

반응 조건

상세 조건
See reaction.notes.procedure_details.

실험 절차

Bacterial cells were harvested by centrifugation and resuspended in SDS sample buffer (0.0625M Tris, pH 6.8, 10% glycerol, 5% mercaptoethanol, 2.3% SDS). The resuspended pellet was boiled for 5 min. and then cleared of insoluble cellular debris by centrifugation. The supernatants obtained from IPTG-induced cultures of GE3-2, GE9-2, GE15-1, GE17-2, GE4-8, EXP3-7, GE1-N and GE57 were analyzed by SDS-polyacrylamide gel electrophoresis (PAGE) together with uninduced lysates. The proteins from these gels were then transferred to nitrocellulose filters (i.e., by Western blotting).

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US05874563uspto-grants-1999_02