반응 #436784

ord-54430c6430da44529262d57f7ce4b87f

반응 방정식

CNC(C)C1CCC(N)C(OC2C(N)CC(N)C(OC3OCC(C)(O)C(NC)C3O)C2O)O1
gentamycin
CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)O.CCN(CC)CCOC(=O)c1ccc(N)cc1.O
penicillin 100
NC(=O)CC[C@H](N)C(=O)O
L-glutamine
O=C=O
CO2
O=S(=O)(O)CCN1CCN(CCO)CC1
HEPES
CCC[C@@H]1C[C@H](OC)C[C@H](C)C[C@@H]2C[C@@H](OC(=O)/C=C\CCc3coc(/C=C\CNC(=O)OC)n3)C[C@H](CC(=O)O1)O2
Neopeltolide

반응 조건

상세 조건
See reaction.notes.procedure_details.

후처리

  1. 1
    온도All cell lines are maintained in Roswell Park Memorial Institute (RPMI) medium 1640
  2. 2
    기타Cell lines are cultured in plastic tissue culture flasks
  3. 3
    기타kept in an incubator at 37° C. in humidified air

실험 절차

All cell lines are maintained in Roswell Park Memorial Institute (RPMI) medium 1640 supplemented with 100 U/mL penicillin 100 μg/ml streptomycin, 60 μg/ml L-glutamine, 18 mM HEPES, 0.05 mg/mL gentamycin and 10% fetal bovine serum. Cell lines are cultured in plastic tissue culture flasks and kept in an incubator at 37° C. in humidified air containing 5% CO2.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US07179828B2uspto-grants-2007_02