반응 #436784
ord-54430c6430da44529262d57f7ce4b87f
반응 방정식
gentamycin
penicillin 100
L-glutamine
CO2
HEPES
→
Neopeltolide
반응 조건
상세 조건
See reaction.notes.procedure_details.
후처리
- 1온도All cell lines are maintained in Roswell Park Memorial Institute (RPMI) medium 1640
- 2기타Cell lines are cultured in plastic tissue culture flasks
- 3기타kept in an incubator at 37° C. in humidified air
실험 절차
All cell lines are maintained in Roswell Park Memorial Institute (RPMI) medium 1640 supplemented with 100 U/mL penicillin 100 μg/ml streptomycin, 60 μg/ml L-glutamine, 18 mM HEPES, 0.05 mg/mL gentamycin and 10% fetal bovine serum. Cell lines are cultured in plastic tissue culture flasks and kept in an incubator at 37° C. in humidified air containing 5% CO2.