반응 #40486

반응 방정식

sodium triacetoxyborohydride

Trifluoroacetic acid

3(S)-(6-chloro-9H-β-carbolin-8-ylcarbamoyl)-morpholine-4-carboxylic acid tert-butyl ester

N-(tert-butoxycarbonyl)-D-prolinal

→

2(R)-[3(S)-(6-Chloro-9H-β-carbolin-8-ylcarbamoyl)-morpholin-4-ylmethyl]-pyrrolidine-1-carboxylic acid tert-butyl ester

수율 76.7%

용매

THF

MeOH CH2Cl2

CH2Cl2

THF

반응 조건

후처리

1. 1
   농축concentrated to a residue
2. 2
   농축The residue was concentrated once more from CH2Cl2
3. 3
   기타to yield a yellow-brown solid which
4. 4
   온도Gentle warming
5. 5
   workup.DISSOLUTIONdissolution
6. 6
   workup.STIRRINGThe solution was stirred at RT for 30 min
7. 7
   기타quenched via addition of 1N aqueous NaOH (30 ml)
8. 8
   workup.ADDITIONThe mixture was poured into a separatory funnel
9. 9
   workup.ADDITIONcontaining EtOAc (100 ml), H2O (100 ml), and brine (100 ml)
10. 10
    workup.STIRRINGThe mixture was shaken
11. 11
    기타the layers were separated
12. 12
    추출The aqueous layer was extracted with EtOAc (2×50 ml)
13. 13
    세척the combined organic layers were washed with brine
14. 14
    기타The organic layer was dried
15. 15
    여과filtered
16. 16
    농축concentrated
17. 17
    기타to yield a light brown solid

실험 절차

A solution of 3(S)-(6-chloro-9H-β-carbolin-8-ylcarbamoyl)-morpholine-4-carboxylic acid tert-butyl ester (1.00 g, 2.32 mmol) in CH2Cl2 (6 ml) was stirred at RT. Trifluoroacetic acid (6 ml) was added and the solution was stirred at RT for 45 min, then concentrated to a residue. The residue was concentrated once more from CH2Cl2 to yield a yellow-brown solid which was dissolved in THF (13 ml) under argon. Gentle warming was sometimes needed to ensure complete dissolution. A solution of N-(tert-butoxycarbonyl)-D-prolinal (693 mg, 3.48 mmol) in THF (2 ml) was added, followed by sodium triacetoxyborohydride (738 mg, 3.48 mmol). The solution was stirred at RT for 30 min, then quenched via addition of 1N aqueous NaOH (30 ml). The mixture was poured into a separatory funnel containing EtOAc (100 ml), H2O (100 ml), and brine (100 ml). The mixture was shaken and the layers were separated. The aqueous layer was extracted with EtOAc (2×50 ml) and the combined organic layers were washed with brine. The organic layer was dried, filtered and concentrated to yield a light brown solid. Column chromatography (2%-4% MeOH/CH2Cl2) yielded 2(R)-[3(S)-(6-Chloro-9H-β-carbolin-8-ylcarbamoyl)-morpholin-4-ylmethyl]-pyrrolidine-1-carboxylic acid tert-butyl ester as a white solid (915 mg).

출처