반응 #347405

반응 방정식

DTNB

Mercaptoethanol

SMCC caproate

pyridine

N-[4-(5-carboxypentylcarbamoyl)-cyclohexylmethyl]-2-(carboxy)ethyl thiosuccinimide

→

N-[4-(5-carboxypentylcarbamoyl)-cyclohexylmethyl]-2-(hydroxy)ethyl thiosuccinimide

반응 조건

후처리

1. 1
   기타the tube sealed
2. 2
   기타the solvents were removed in vacuo
3. 3
   workup.DISSOLUTIONThe residue was dissolved in 0.03 ml of 0.2M potassium phosphate

실험 절차

Mercaptoethanol (0.0045 ml of 1.43M solution in dimethylformamide, 6.4×10-6 mol) was added to SMCC-caproate (0.016 ml, 6.4×10-6 mol) in pyridine (see Example 6). The solution was gassed with argon and the tube sealed. After 12 hr at room temperature, no thiol remained (as determined by reaction with DTNB) and the solvents were removed in vacuo. The residue was dissolved in 0.03 ml of 0.2M potassium phosphate, pH 7, to make a 213 mM solution of the desired compound (see FIG. 1D).

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