반응 #345451

반응 방정식

[3H]spiperone

(+)-butaclamol

Tris HCl

NaCl

MgCl2

→

Dopamine

반응 조건

후처리

1. 1
   기타0.5 mL aliquots of the membrane preparation
2. 2
   여과samples were filtered rapidly through Whatman GF/C glass filters under negative pressure
3. 3
   세척washed three times with ice-cold binding buffer (5 mL)

실험 절차

0.5 mL aliquots of the membrane preparation were incubated with unlabeled drugs, and 0.15 nM [3H]spiperone in a final volume of 1 mL containing 50 mm Tris HCl, 120 mM NaCl and 1 mM MgCl2 (pH 7.7). Nonspecific binding was measured in the presence of 100 nM (+)-butaclamol. After 15 minutes of incubation at 37° C., samples were filtered rapidly through Whatman GF/C glass filters under negative pressure, and washed three times with ice-cold binding buffer (5 mL).

출처