반응 #341216

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TBTU

N-{[4-((2R,3R)-1-(4-Fluorophenyl)-3-{[2-(4-fluorophenyl)-2-oxoethyl]thio}-4-oxoazetidin-2-yl)phenoxy]acetyl}-D-valine

tert-butyl L-methioninate hydrochloride

N-methylmorpholine

sodium borohydride

Ammonium acetate

tert-butyl L-methioninate hydrochloride

N-methylmorpholine

TBTU

→

title compound

N-{[4-((2R,3R)-1-(4-fluorophenyl)-3-([2-(4-fluorophenyl)-2-hydroxyethyl]thio}-4-oxoazetidin-2-yl)phenoxy]acetyl}-D-valyl-L-methionine

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후처리

1. 1
   workup.STIRRINGthe mixture was stirred for 2 h
2. 2
   기타The yellow suspension was purified on silica gel (1 g)
3. 3
   세척eluted with EtOAc:DCM (15:85)
4. 4
   농축The pure fractions were concentrated
5. 5
   workup.ADDITIONformic acid (1.5 ml) was added
6. 6
   workup.STIRRINGThe solution was stirred at 50° C. overnight
7. 7
   기타The solvent was removed under reduced pressure
8. 8
   workup.ADDITIONToluene was added
9. 9
   기타removed under reduced pressure
10. 10
    workup.DISSOLUTIONThe residue was dissolved in methanol (1 ml)
11. 11
    기타The resulting reaction mixture
12. 12
    workup.STIRRINGwas stirred for 10 minutes
13. 13
    기타the solvent was removed under reduced pressure
14. 14
    기타The residue was purified with preparative HPLC on a C8 column

실험 절차

N-{[4-((2R,3R)-1-(4-Fluorophenyl)-3-{[2-(4-fluorophenyl)-2-oxoethyl]thio}-4-oxoazetidin-2-yl)phenoxy]acetyl}-D-valine (13.3 mg, 0.023 mmol), tert-butyl L-methioninate hydrochloride (7.4 mg, 0.031 mmol) and N-methylmorpholine (10 μl, 0.091 mmol) were dissolved in 1 ml. After 5 minutes, TBTU (8.9 mg, 0.028 mmol) was added and the resulting suspension was stirred overnight. Additional tert-butyl L-methioninate hydrochloride (2.1 mg, 0087 mmol), N-methylmorpholine (5 μl, 45 μmol) and TBTU (2.1 mg, 6.54 μmol) were added and the mixture was stirred for 2 h. The formation of the ester was confirmed. MHz 768.1 (M−H) and 770.0 (M+H). The yellow suspension was purified on silica gel (1 g) and eluted with EtOAc:DCM (15:85). The pure fractions were concentrated and formic acid (1.5 ml) was added. The solution was stirred at 50° C. overnight. The solvent was removed under reduced pressure. Toluene was added and removed under reduced pressure. The residue was dissolved in methanol (1 ml) and sodium borohydride (9.9 mg, 0.26 mmol) was added. The resulting reaction mixture was stirred for 10 minutes. Ammonium acetate (18.9 mg) was added and the solvent was removed under reduced pressure. The residue was purified with preparative HPLC on a C8 column. A gradient from 20% to 40% MeCN in 0.1 M ammonium acetate buffer was used as eluent. After lyophilisation, the title compound was obtained as a white solid was obtained (4.6 mg, 28%). 1H-NMR (400 MHz, DMSO-d6): 0.75 (d, 3H), 0.79 (d, 3H), 1.79-1.97 (m, 3H), 1.99 (s, 3H), 2.36-2.44 (m, 2H), 2.86-2.92 (m, 2H), 2.24-4.35 (m, 3H), 4.58 (d, 2H), 4.67-4.76 (m, 1H), 5.03 (d, 0.5H), 5.5 (d, 0.5H), 5.63 (t, 1H), 6.95 (d, 2H), 7.05-7.16 (m, 4H), 7.18-7.24 (m, 2H), 7.30-7.38 (m, 2H), 7.82 (d, 1H), 7.37 (d, 1H). M/z: 714.0 (M−1) and 716.1 (M+H).

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