반응 #317563

반응 방정식

2-[1(S)-{(1,1-Dimethylethoxy)carbonylamino}-2-phenylethyl]-1-(2-oxo-butyl)-4-phenyl-1H-imidazole

→

tetrahydro-imidazo-pyrazine

수율 86.0%

용매

TFA

DCM

TFA

반응 조건

후처리

1. 1
   농축concentrated under reduced pressure
2. 2
   workup.DISSOLUTIONThe resulting intermediate dihydro-imidazo-pyrazine was dissolved in methanol
3. 3
   workup.ADDITIONwas added
4. 4
   workup.STIRRINGAfter about 2 hours of stirring at about 20° C.
5. 5
   여과the mixture was filtered
6. 6
   농축the filtrate concentrated under reduced pressure
7. 7
   기타The residue was purified by flash chromatography (ethyl acetate/Heptane 7:3; Rf=0.30)

실험 절차

2-[1(S)-{(1,1-Dimethylethoxy)carbonylamino}-2-phenylethyl]-1-(2-oxo-butyl)-4-phenyl-1H-imidazole (60 mg) in a mixture of 10% TFA in DCM was stirred at about 20° C. for about 3 hours and then concentrated under reduced pressure. The resulting intermediate dihydro-imidazo-pyrazine was dissolved in methanol and borohydride supported on resin (AMBERLITE® IRA 400, Aldrich, 2.5 mmol BH4−/g; 4 eq) was added. The pH was maintained at about 5 by addition of drops of TFA. After about 2 hours of stirring at about 20° C., the mixture was filtered and the filtrate concentrated under reduced pressure. The residue was purified by flash chromatography (ethyl acetate/Heptane 7:3; Rf=0.30). The tetrahydro-imidazo-pyrazine was obtained as a single diastereoisomer in 86% yield (38 mg) NMR (1H, 400 MHz, CDCl3) δ: 7.87-7.10 (m, 11H, arom, H), 4.28 (dd, 1H, 3J=10 Hz, 3J=3 Hz, H8), 3.95 (dd, 1H, 2J=11.5 Hz, 3J=3.6 Hz), 3.85 (dd, 1H, 2J=13.6 Hz, 3J=3.0 Hz), 3.60 (t, 1H, 2J=3J=11.5 Hz), 3.85 (dd, 1H, 2J=13.6 Hz, 3J=10, Hz), 2.98 (m, 2H), 1.85 (s, 1H, NH), 1.55 (m, 2H, CH2), 0.95 (t, 3H, CN3). NMR (13C, 100 MHz CDCl3): 146.3, 140.9, 138.0, 134.4, 129.4, 128.6, 128.5, 126.6, 126.5, 124.8, 113.8, 55.9, 54.4, 50.2, 40.0, 26.6, 10.0. LC/MS: calculated MW=317.43, m/z=318.20 (M+H).

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