반응 #307520

ord-7db65914d01d4f049bfc3bd1369acb02

반응 방정식

NNC(=O)c1ccncc1
isoniazid
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC[C@@H](CO)NCCN[C@@H](CC)CO
ethambutol
NC(=O)c1cnccn1
Pyrazinamide
CO[C@H]1/C=C/O[C@@]2(C)Oc3c(C)c(O)c4c(O)c(c(/C=N/N5CCN(C)CC5)c(O)c4c3C2=O)NC(=O)/C(C)=C\C=C\[C@H](C)[C@H](O)[C@@H](C)[C@@H](O)[C@@H](C)[C@H](OC(C)=O)[C@@H]1C
rifampicin
NNC(=O)c1ccncc1
isoniazid
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC[C@@H](CO)NCCN[C@@H](CC)CO
ethambutol
CC(C)=CCC/C(C)=C/CNCCNC1C2CC3CC(C2)CC1C3
SQ109

용매

반응 조건

온도
-80°CELSIUS
상세 조건
See reaction.notes.procedure_details.

후처리

  1. 1
    workup.DISTILLATIONin distilled
  2. 2
    여과deionized water at 10 mg/mL, sterilized by filtration
  3. 3
    기타Stock solutions of rifampicin, at 1 or 10 mg/mL, were prepared in methanol
  4. 4
    기타stored at −80° C
  5. 5
    기타, and a stock solution was prepared
  6. 6
    기타stored at 80° C.

실험 절차

Antimicrobial drugs isoniazid, rifampicin, streptomycin, and ethambutol were purchased from Sigma-Aldrich. St. Louis, Mo. Stock solutions of isoniazid, streptomycin, and ethambutol were prepared in distilled and deionized water at 10 mg/mL, sterilized by filtration, and stored frozen at −80° C. Stock solutions of rifampicin, at 1 or 10 mg/mL, were prepared in methanol and stored at −80° C. Pyrazinamide was purchased as the drug reconstituting kit from Becton Dickinson, Cockeysville, Md., and a stock solution was prepared following instructions by the manufacturer. Stock solutions of SQ109 were prepared in methanol at 1 mg/mL and stored at 80° C.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US08202910B2uspto-grants-2012_06