반응 #2605

반응 방정식

sodium bicarbonate

Boc-L-alanine

4-Aminomethyl-N-{2-[4-(4-fluorobenzoyl)piperidino]ethyl}-N-(2-methoxyethyl)benzamide

EDC hydrochloride

→

title compound

수율 72.6%

4-[(S)-2-(tert-Butoxycarbonylamino)propionyl]aminomethyl-N-{2-[4-(4-fluorobenzoyl)piperidino]ethyl}-N-(2-methoxyethyl)benzamide

수율 72.6%

용매

water

methylene chloride

반응 조건

후처리

1. 1
   온도while cooling in an ice bath
2. 2
   추출extracted with methylene chloride
3. 3
   건조Thereafter, the resulting organic layer was dried on anhydrous sodium carbonate
4. 4
   기타the solvent was removed by evaporation
5. 5
   기타the resulting residue was purified by a silica gel column chromatography (ethyl acetate)

실험 절차

4-Aminomethyl-N-{2-[4-(4-fluorobenzoyl)piperidino]ethyl}-N-(2-methoxyethyl)benzamide (120 mg, 0.245 mmol) was dissolved in methylene chloride (5 ml) to which were subsequently added Boc-L-alanine (46.4 mg, 0.245 mmol) and EDC hydrochloride (52 mg, 0.270 mmol) while cooling in an ice bath. After 4 hours of stirring at the same temperature, the reaction solution was mixed with water (10 ml), adjusted to pH 8 with saturated sodium bicarbonate aqueous solution and then extracted with methylene chloride. Thereafter, the resulting organic layer was dried on anhydrous sodium carbonate, the solvent was removed by evaporation, and the resulting residue was purified by a silica gel column chromatography (ethyl acetate) to obtain 109 mg (68.9%) of the title compound in a colorless amorphous form.

출처