반응 #2507845

ord-6c8e633799a94f37a39153d5f8c3caac

반응 방정식

O.O.OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
trehalose dihydrate
CC(C[*:1])[*:2]
polypropylene
Cl
HCl
→
OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
Trehalose

반응물

O.O.OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
trehalose dihydrate
CC(C[*:1])[*:2]
polypropylene
Cl
HCl

시약

없음

용매

O
water
O
water

반응 조건

상세 조건
See reaction.notes.procedure_details.

후처리

  1. 1
    workup.ADDITIONwas added at room temperature
  2. 2
    기타was obtained
  3. 3
    기타to obtain 400 ml final volume
  4. 4
    여과The solution was filtered through a 0.22-micron
  5. 5
    여과filter
  6. 6
    workup.ADDITIONto dilute the protein solution

실험 절차

25.48 g of trehalose dihydrate was carefully weighed and transferred into a sterile polypropylene bottle, to which 350 ml of purified water was added at room temperature and stirred slowly until a clear solution was obtained. To the clear solution, 0.1N HCl was added drop by drop to adjust the pH to 3.9, then the volume was adjusted with purified water to obtain 400 ml final volume. The pH was measured and found to be 4.2. The solution was filtered through a 0.22-micron filter and was used directly to dilute the protein solution.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US07956028B2uspto-grants-2011_06