반응 #2201138

반응 방정식

[4-(2-amino-4-methyl-thiazol-5-yl)-pyrimidin-2-yl]-(3-nitro-phenyl)-amine

Boc-βAla-OH

1,3-diisopropylcarbodiimide

DMF

→

({4-Methyl-5-[2-(3-nitro-phenylamino)-pyrimidin-4-yl]-thiazol-2-ylcarbamoyl}-methyl)-carbamic acid tert-butyl ester

반응 조건

후처리

1. 1
   추출extracted with EtOAc
2. 2
   세척washed with brine
3. 3
   여과filtered
4. 4
   기타dried on MgSO4
5. 5
   기타The solvent was evaporated
6. 6
   기타to give brown residue, which
7. 7
   기타was purified by SiO2 chromatography (1:1 EtOAc/PE)

실험 절차

A mixture of [4-(2-amino-4-methyl-thiazol-5-yl)-pyrimidin-2-yl]-(3-nitro-phenyl)-amine (0.12 g, 0.37 mmol), Boc-βAla-OH (0.18 g, 0.93 mmol), 1,3-diisopropylcarbodiimide (0.07 mL, 0.45 mmol) and 4-N,N-dimethylaminopyridine (36 mg, 0.3 mmol) in of dry DMF (2 mL) was stirred at room temperature for 24 h. The reaction mixture was poured into ice water and extracted with EtOAc. The organics were combined and washed with brine, filtered, and dried on MgSO4. The solvent was evaporated to give brown residue, which was purified by SiO2 chromatography (1:1 EtOAc/PE) to afford ({4-Methyl-5-[2-(3-nitro-phenylamino)-pyrimidin-4-yl]-thiazol-2-ylcarbamoyl}-methyl)-carbamic acid tert-butyl ester as light yellow solid. Anal. RP-HPLC: tR=19.5 min (0-60% MeCN in 0.1% aq CF3COOH over 20 min, 1 mL/min, purity>93%). 1H-NMR (DMSO-d6) δ: 1.11 (s, 9H, CH3), 2.54 (s, 3H, CH3), 3.62 (m, 2H, CH2), 5.38 (m, 2H, CH2), 7.06 (d, 1H, J=5.5 Hz, pyrimidinyl-H), 7.46 (m, 1H, Ph-H), 7.72 (m, 1H, Ph-H), 8.15 (m, 1H, Ph-H), 8.45 (d, 1H, J=5.2 Hz, pyrimidinyl-H), 8.77 (s, 1H, NH). A solution of this material (97 mg, 0.19 mmol) in dioxane (5 mL) was treated with CF3COOH (1 ml). After stirring at room temperature for 22 h, the reaction mixture was evaporated and purified by preparative RP-HPLC (0-60% MeCN in 0.1% aq CF3COOH over 40 min, 9 mL/min) to afford the titled compound (34 mg) as a light yellow solid. Anal. RP-HPLC: tR=14.0 min (0-60% MeCN in 0.1% aq CF3COOH over 20 min, 1 mL/min, purity>93%). 1H-NMR (CD3OD) δ: 2.64 (s, 3H, CH3), 3.30 (m, 2H, CH2), 3.33 (m, 2H, CH2), 7.14 (d, 1H, J=5.5 Hz, pyrimidinyl-H), 7.52 (t, 1H, J=8.2 Hz, Ph-H), 7.63 (m, 1H, Ph-H), 7.96 (m, 1H, Ph-H), 8.46 (d, 1H, J=5.5 Hz, pyrimidinyl-H), 8.88 (s, 1H, NH). MS (ESi+) m/z 400.6 (C17H17N7O3S requires 399.4).

출처