반응 #217754

반응 방정식

2,6-Diamino-9-(2-fluoro-β-D-arabinosyl)purine

adenosine

→

title compound

수율 100.0%

9-(2-Fluoro-β-D-arabinosyl)guanine

수율 100.0%

용매

Tris-HCl

반응 조건

후처리

1. 1
   기타The resultant reaction mixture
2. 2
   여과was subjected to filtration by use of a membrane
3. 3
   여과filter (PTFE, 0.5 μm)
4. 4
   기타the filtrate was prepared into 60 mL of an aqueous solution
5. 5
   기타The aqueous solution was purified by means of reverse-phase ODS column chromatography (80 mL, 0 to 2% aqueous acetonitrile)

실험 절차

2,6-Diamino-9-(2-fluoro-β-D-arabinosyl)purine (61.2 mg, 0.21 mmol) was dissolved in 50 mM Tris-HCl buffer solution (20 mL, pH 7.0), and adenosine deaminase (71 units) was added to the resultant solution, followed by stirring at room temperature for 1.5 hours. The resultant reaction mixture was subjected to filtration by use of a membrane filter (PTFE, 0.5 μm), and the filtrate was prepared into 60 mL of an aqueous solution. The aqueous solution was purified by means of reverse-phase ODS column chromatography (80 mL, 0 to 2% aqueous acetonitrile), to thereby yield 60.4 mg of the title compound as colorless crystals (yield: 100%).

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