반응 #2054

ord-ca050bcf3e3041878a46906ef366ed6a

반응 방정식

C[C@H](N)C(=O)O
L-alanine
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
C[C@H](N)C(=O)O
L-alanine
→
N[C@H](Cc1ccccc1)C(=O)O
D-phenylalanine
N[C@@H](Cc1ccccc1)C(=O)O
L-phenylalanine

반응물

C[C@H](N)C(=O)O
L-alanine
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
C[C@H](N)C(=O)O
L-alanine

시약

없음

반응 조건

상세 조건
See reaction.notes.procedure_details.

후처리

  1. 1
    기타over 52 hours
  2. 2
    농축In addition, L-phenylalanine was fed at the same concentration and rate as the D-, L-alanine

실험 절차

The amount of glucose fed was 2021 g over 52 hours. The fermentation medium included a D-, L-alanine feed whereby a total of 1400 mls of 167 g/l D-, L-alanine was fed at a rate of 1.9 ml/min starting 12 hrs from the beginning of the fermentation. In addition, L-phenylalanine was fed at the same concentration and rate as the D-, L-alanine. The fermentation resulted in 13.66 g/l of D-phenylalanine and 0.87 g/l L-phenylalanine being produced.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US05728555uspto-grants-1998_03