반응 #2053
ord-5b62ce49ffab4c1db9e358eac8f40d5a
반응 조건
실험 절차
Using cultures of recombinant cells of the present invention with the addition of D-, L-alanine and L-phenylalanine as additional sources of D-alanine and phenylpyruvate substrates for the D-aminotransferase gene product resulted in the production of 13.66 g/l of D-phenylalanine and 0.47 g/l of L-phenylalanine, a 94% enantiomeric excess. In the case where only D-, L-alanine was added to the cultures during the fermentation process resulted in the production of 4.15 g/l of D-phenylalanine and no L-phenylalanine, a 100% enantiomeric excess. In contrast, when no D-, L-alanine or L-phenylalanine was added to the cell cultures during the fermentation process, 1.12 g/l of D-phenylalanine and 0.47 g/l of L-phenylalanine is produced, a 41% enantiomeric excess.