반응 #2045076

ord-8c1e422eb42a45e3ad29072cd8066b41

반응 방정식

[Na+].[OH-]
NaOH
O=C[C@H](O)[C@@H](O)[C@H](O)CO
xylose
O=Cc1ccco1
furfural
OC[C@@H](O)[C@H](O)[C@@H](O)CO
xylitol

시약

없음

반응 조건

상세 조건
See reaction.notes.procedure_details.

후처리

  1. 1
    기타obtained by a pretreatment process of acid-catalyzed steam explosion
  2. 2
    기타is 180° C.
  3. 3
    workup.ADDITIONThe reaction mix
  4. 4
    기타is then separated by a solid-liquid separation equipment
  5. 5
    기타to get the liquid fraction as hydrolysate
  6. 6
    기타is controlled at 30° C.
  7. 7
    온도the agitation of the incubator is maintained at 100 rpm
  8. 8
    workup.WAITafter 40 hr of fermentation

실험 절차

The fermentation medium is napiergrass hydrolysate obtained by a pretreatment process of acid-catalyzed steam explosion. Pretreatment conditions: the concentration of the dilute acid is 2%, the operation temperature is 180° C., the reaction time at this high temperature is 5 min, after which the reactor is immediately depressurized. The reaction mix is then separated by a solid-liquid separation equipment to get the liquid fraction as hydrolysate. In this case, NaOH is added into the napiergrass hydrolysate to adjust to pH 6.0, the fermentation temperature is controlled at 30° C., the agitation of the incubator is maintained at 100 rpm, the ratio of the inocula size of the culture and the volume of the fermentation medium is 1:6 (v/v). The content of xylose in the napiergrass hydrolysate is 15.55 g/L, the content of the inhibitor furfural is 1.19 g/L, and after 40 hr of fermentation, the amount of xylitol produced is 5.11 g/L, and the overall utilization of xylose is up to 95.2%, as shown in Table 5.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US08409835B2uspto-grants-2013_04