반응 #2006451

반응 방정식

sodium hydroxide

N-Boc-3-pyrrolidinone

D-glucose

D-alanine

pyridoxal phosphate

N-Boc-3-pyrrolidinone

D-glucose

NAD+

D-alanine

pyridoxal phosphate

→

(R)—N-Boc-3-aminopyrrolidine

반응 조건

후처리

1. 1
   기타(pNTASPAG), obtained in Example 11, which
2. 2
   workup.ADDITIONwas added so that total volume was 25 ml
3. 3
   workup.ADDITIONwere added

실험 절차

To a flask containing 0.5 g of a substrate N-Boc-3-pyrrolidinone, 0.73 g of D-glucose, 4 mg of NAD+, 1.44 g of D-alanine, and 3.3 mg of pyridoxal phosphate, a culture fluid of the recombinant E. coli HB101 (pNTASPAG), obtained in Example 11, which expresses TAS, PALDH, and GDH was added so that total volume was 25 ml. The resultant product was stirred at 30° C. while being adjusted to a pH of 6.8 by drippage of 5 N aqueous solution of sodium hydroxide. After nine hours in reaction, 0.25 g of N-Boc-3-pyrrolidinone, 0.37 g of D-glucose, 0.37 g of D-alanine, and 3 mg of pyridoxal phosphate were added.

출처