반응 #1835894

ord-55c83ba394024cfdb19a1a24bbb667c8

반응 방정식

O=C(O)/C=C/c1cnc[nH]1
urocanate
O=P([O-])([O-])OP(=O)([O-])[O-]
pyrophosphate
N[C@@H](CCC(=O)N[C@@H](CS)C(=O)NCC(=O)O)C(=O)O
glutathione
→
N.N[C@@H](Cc1c[nH]cn1)C(=O)O
Histidine Ammonia

반응물

O=C(O)/C=C/c1cnc[nH]1
urocanate
O=P([O-])([O-])OP(=O)([O-])[O-]
pyrophosphate
N[C@@H](CCC(=O)N[C@@H](CS)C(=O)NCC(=O)O)C(=O)O
glutathione

시약

[Cl-].[Cl-].[Zn+2]
ZnCl2

용매

N[C@@H](Cc1c[nH]cn1)C(=O)O
histidine

반응 조건

상세 조건
See reaction.notes.procedure_details.

후처리

  1. 1
    workup.WAITmonitored for approximately 5 minutes

실험 절차

HAL enzyme activity can be determined as described in Baedeker & Schulz (Eur. J. Biochem 2002, 269, 1790-1797), wherein enzyme activity was determined as the rate of urocanate formation, measured spectrophotometrically at 277 nm. For a standard assay, the enzyme was preincubated at 25° C. for 5 min in 2.5 mL buffer containing 0.1 M pyrophosphate (pH 9.3), 10 μM ZnCl2 and 2 mM glutathione. The reaction was started by adding 200 μL of 0.5 M histidine solution and then monitored for approximately 5 minutes.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US09150845B2uspto-grants-2015_10