반응 #1769598

반응 방정식

adipamide

adiponitrile

5-Cyanovaleramide

5-cyanovalerate

adipamate

adipate

→

ammonium adipate

cyanovalerate

용매

potassium phosphate

반응 조건

후처리

1. 1
   workup.ADDITIONA cellular residue containing 13.1 mg of dry weight of cells
2. 2
   기타was carried out at 25° C.
3. 3
   workup.ALIQUOTby sampling

실험 절차

A cellular residue containing 13.1 mg of dry weight of cells was suspended in 2 ml of a 52.3 mM solution of adiponitrile in 50 mM potassium phosphate buffer, pH 7. The reaction was carried out at 25° C., with shaking, and the kinetics were followed by sampling. 5-Cyanovaleramide, adipamide, 5-cyanovalerate, adipamate and adipate were determined on each sample by high performance liquid chromatography (HPLC). The results are collated in FIG. 1, which shows the curves of the yield (on the ordinate) of cyanovalerate (curve a) and ammonium adipate (curve b). The respective rates of formation of cyanovalerate and adipate were greater than 0.45 and equal to 0.15 U/mg of dry weight of cells (1 U is equal to 1 μmol of product formed per minute).

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