반응 #1702032

반응 방정식

irinotecan hydrochloride trihydrate

sodium

cysteic acid

→

irinotecan

용매

Methanol

반응 조건

후처리

1. 1
   기타evaporated in vacuo
2. 2
   workup.ADDITION100 ml of water was added to the residue
3. 3
   기타obtained
4. 4
   기타after evaporation
5. 5
   기타transparent (approx. 30 min)
6. 6
   여과The obtained solution was filtered through 0.2 mm
7. 7
   여과filter
8. 8
   기타freeze dried
9. 9
   여과The filtration

실험 절차

Methanol stock-solutions of irinotecan hydrochloride trihydrate (100 ml, 1.15 mg/ml) and sodium salt of methyl ester of N-all-trans-retinoyl cysteic acid (27 ml, 15 mg/ml) were mixed in a 500 ml round-bottom flask and evaporated in vacuo. 100 ml of water was added to the residue obtained after evaporation and the mixture was stirred until it became clear and transparent (approx. 30 min). The concentration of irinotecan in the obtained solution was 1 mg/ml, corresponding to an irinotecan hydrochloride trihydrate concentration of 1.15 mg/ml. The obtained solution was filtered through 0.2 mm filter and freeze dried. The filtration did not result in reduction of irinotecan concentration.

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