반응 #1702031

반응 방정식

topotecan hydrochloride

sodium

cysteic acid

→

topotecan

용매

Methanol

반응 조건

후처리

1. 1
   기타evaporated in vacuo
2. 2
   workup.ADDITION120 ml sodium chloride solution (9 mg/ml) was added to the residue
3. 3
   기타obtained
4. 4
   기타after evaporation
5. 5
   기타transparent (approx. 20 min)
6. 6
   기타The solution obtained
7. 7
   여과was filtered through 0.2 mm
8. 8
   여과filter
9. 9
   여과The filtration

실험 절차

Methanol stock-solutions of topotecan hydrochloride (120 ml, 1.09 mg/ml) and sodium salt of methyl ester of N-all-trans-retinoyl cysteic acid (32 ml, 15 mg/ml) were mixed in a 500 ml round-bottom flask and evaporated in vacuo. 120 ml sodium chloride solution (9 mg/ml) was added to the residue obtained after evaporation, and the mixture was stirred until it became clear and transparent (approx. 20 min). The concentration of topotecan in the obtained solution was 1 mg/ml, corresponding to a topotecan hydrochloride concentration of 1.09 mg/ml. The solution obtained was filtered through 0.2 mm filter. The filtration did not result in reduction of topotecan concentration.

출처