반응 #1664797

ord-e2386fd5b9504ea59d6f445405e5d560

반응 방정식

O=C([O-])C[C@H](O)C(=O)[O-]
L-malate
NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
NAD+
→
CC(O)C(=O)[O-]
lactate
O=C([O-])CC(O)C(=O)[O-]
malate

반응물

O=C([O-])C[C@H](O)C(=O)[O-]
L-malate
NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
NAD+
—
MnCl2

시약

없음

용매

O=P([O-])([O-])[O-]
phosphate

반응 조건

상세 조건
See reaction.notes.procedure_details.

후처리

  1. 1
    기타4 g dry weight of cells immobilized

실험 절차

4 g dry weight of cells immobilized as described in Example 17 were added to 50 ml 25 mM L-malate in 0.1 M phosphate of pH 5.0 together with 85 μmol NAD+ and 35 μmol MnCl2. Total conversion of malate to lactate was obtained in 3 hrs. The procedure could be repeated at least five times without any increase in reaction time.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US04288552uspto-grants-1981_09