반응 #1563321

반응 방정식

methyl-[1-(4-chloro-3-{[(3R)-2-(4-chlorophenyl)-4,4,4-trifluoro-3-methylbutanoyl]amino}phenyl)cyclobutyl]acetate

sodium hydroxide

hydrochloric acid

→

[1-(4-Chloro-3-{[(3R)-2-(4-chlorophenyl)-4,4,4-trifluoro-3-methylbutanoyl]amino}-phenyl)cyclobutyl]acetic acid

용매

dioxane

반응 조건

후처리

1. 1
   추출extracted repeatedly with ethyl acetate
2. 2
   세척The combined organic phases were washed with saturated sodium chloride solution
3. 3
   건조dried over sodium sulphate
4. 4
   농축concentrated under reduced pressure
5. 5
   기타The crude product was purified by preparative HPLC

실험 절차

38 mg (0.08 mmol) of methyl-[1-(4-chloro-3-{[(3R)-2-(4-chlorophenyl)-4,4,4-trifluoro-3-methylbutanoyl]amino}phenyl)cyclobutyl]acetate were dissolved in 9.5 ml of dioxane, and 0.15 ml of 1 N aqueous sodium hydroxide solution was added. The mixture was stirred at 80° C. overnight. The reaction mixture was then acidified with 1 N hydrochloric acid to pH 1 and extracted repeatedly with ethyl acetate. The combined organic phases were washed with saturated sodium chloride solution, dried over sodium sulphate and concentrated under reduced pressure. The crude product was purified by preparative HPLC. This gave 22 mg (0.05 mmol, 60% of theory) of the target compound.

출처