반응 #1509610

반응 방정식

2-[4,7-bis(1,4-dicarboxybutyl]-1,4,7,10-tetraazacyclododec-1-yl]hexanoic acid

NaOH

HCl

sulfo-NHS

EDCI

3-aminopropane-1,2-diol

→

product

DO3A

용매

water

반응 조건

후처리

1. 1
   기타is prepared
2. 2
   농축the reaction medium is concentrated to approximately 2 ml
3. 3
   기타precipitated from 10 ml of ethanol
4. 4
   여과The solid is filtered off
5. 5
   세척washed with ethanol and diethyl ether
6. 6
   기타purified on silanized silica RP2, elution

실험 절차

A solution containing 0.26 mg of 3-aminopropane-1,2-diol in 6 μl of water is prepared. The pH is adjusted to 6 with HCl. 0.5 mg of gallium complex of 2-[4,7-bis(1,4-dicarboxybutyl]-1,4,7,10-tetraazacyclododec-1-yl]hexanoic acid are added to the above solution. The pH is again adjusted before adding 0.071 mg of sulfo-NHS and 0.062 mg of EDCI. The pH is checked and adjusted to 6 with 2N NaOH. After an overnight period at AT, the reaction medium is concentrated to approximately 2 ml and then precipitated from 10 ml of ethanol. The solid is filtered off, washed with ethanol and diethyl ether, and then purified on silanized silica RP2, elution being carried out with water only. 0.2 mg of product is obtained.

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