반응 #1500373

반응 방정식

adenosine triphosphate

phosphoenol pyruvate

pyruvate

cephalosporin C

cephalosporin C

→

Isopenicillin N

반응 조건

후처리

1. 1
   추출extract
2. 2
   기타produced in accordance with the procedure
3. 3
   기타produces the same size inhibitory zone

실험 절차

Five μmoles of adenosine triphosphate (ATP), 10 μmoles of phosphoenol pyruvate, and 100 μgrams of pyruvate kinase were incubated for three hours at 25° C. at a pH of 7.2 with 100 μg of "LLD" and 1.0 ml of mutant M-0198 extract produced in accordance with the procedure set forth above. The reaction mixture showed the presence of antibiotic activity equivalent to 2.3 μg/ml using the β-lactam antibiotic, cephalosporin C, as standard i.e. 2.3 units/ml. One unit of antibiotic activity is that which produces the same size inhibitory zone as does 1 μg of cephalosporin C.

출처