반응 #1483117

반응 방정식

Oxytocin acetate

disodium pamoate

→

Oxytocin

수율 79.0%

용매

EtOAc

MeOH

반응 조건

후처리

1. 1
   workup.ADDITIONadded to the polymer solution
2. 2
   기타yielding a milky suspension as the organic phase
3. 3
   기타to provide an emulsion
4. 4
   기타The emulsion was collected directly into a 0.3% PVA solvent extraction solution (150 mL)
5. 5
   추출to extract EtOAc
6. 6
   여과Hardened microparticles were collected by filtration
7. 7
   세척washed with water, air
8. 8
   기타dried
9. 9
   기타stored at 4° C

실험 절차

PLGA polymer (MW 24,000, 180 mg) was dissolved in EtOAc (1800 μL). Oxytocin acetate (40 mg) was dissolved in MeOH (200 μL) and added to the polymer solution yielding a milky suspension as the organic phase. The resulting organic phase was combined with a 1% PVA aqueous phase containing 10 mM disodium pamoate to provide an emulsion. The emulsion was collected directly into a 0.3% PVA solvent extraction solution (150 mL) and stirred for four hours to extract EtOAc. Hardened microparticles were collected by filtration, washed with water, air dried and stored at 4° C. This provided formulation BM (158 mg, 79.0% yield) with a median particle size 144 μm. The core load (8.9%), encapsulation efficiency (44.5%) and in vitro burst (21.1%) were determined by RP-HPLC assay.

출처