반응 #1265349

반응 방정식

histidine

sodium chloride

→

histidine sodium chloride

용매

water

반응 조건

후처리

1. 1
   기타Ethanol was removed from the liposome suspension by diafiltration
2. 2
   여과The solution was filtered through a 0.22 μm
3. 3
   여과Durapore filter
4. 4
   workup.ADDITIONThe liposome suspension was diluted in approximately a 1:1 (v/v) ratio with the histidine/sodium chloride solution
5. 5
   기타to remove the ethanol

실험 절차

Ethanol was removed from the liposome suspension by diafiltration. A histidine/sodium chloride solution was prepared by dissolving histidine (10 mM) and sodium chloride (150 mM) in sterile water. The pH of the solution was adjusted to approximately 7. The solution was filtered through a 0.22 μm Durapore filter. The liposome suspension was diluted in approximately a 1:1 (v/v) ratio with the histidine/sodium chloride solution and diafiltered through a polysulfone hollow-fiber ultrafilter. Eight volume exchanges were performed against the histidine/sodium chloride solution to remove the ethanol. The process fluid temperature was maintained at about 20-30° C. Total diafiltration time was approximately 4.5 hours.

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