반응 #1178409

반응 방정식

sodium formate

acetophenone

→

(S)-phenylethanol

수율 96.4%

용매

formic acid

반응 조건

후처리

1. 1
   기타Culture fluid was obtained
2. 2
   workup.ADDITIONwas added dropwise to the mixture
3. 3
   기타After the reaction
4. 4
   추출the reaction mixture was extracted with toluene
5. 5
   건조was dried over anhydrous sodium sulfate
6. 6
   기타Removal of sodium sulfate, and evaporation of organic solvents from the organic layer under reduced pressure

실험 절차

Culture fluid was obtained by cultivating E. coli HB101 (pNCMFT) in a similar manner to Example 9. In a 100-mL portion of the culture fluid, 2.8 g of sodium formate, 3 mg of NAD+, and 10 g of acetophenone were added, and the mixture was stirred at 30° C. for 20 hours while controlling the pH of the reaction mixture to 6.0 with 5 N aqueous formic acid, which was added dropwise to the mixture. After the reaction, the reaction mixture was extracted with toluene, and combined organic layer was dried over anhydrous sodium sulfate. Removal of sodium sulfate, and evaporation of organic solvents from the organic layer under reduced pressure yielded 9.8 g of (S)-phenylethanol. The product (S)-phenylethanol was analyzed in accordance with the [Analysis condition (1) for gas chromatography] as described above, and found that the optical purity of the product was 99.9% e.e. or higher.

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