반응 #1154364
ord-2de7dc0003074e959375b22cd35d64da
반응 조건
후처리
- 1기타The linker product was purified by column chromatography on silica gel
- 2세척The resulting linker-bound substrate was thoroughly rinsed with pure toluene and methylene chloride
- 3기타dried for about 18 hours under reduced pressure
실험 절차
Binder DNA oligonucleotide-derivatized porous silicon films were employed to test the selectivity and limits of detection of a contemplated sensor. For attachment of DNA, a trimethoxy-3-bromoacetamido-propylsilane linker was synthesized by reaction of bromoacetic acid with trimethoxy-(3-aminopropyl)silane in the presence of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide-hydrochloride in methylene chloride as solvent. The linker product was purified by column chromatography on silica gel. The oxidized porous silicon samples were then contacted with a toluene solution of the linker for 2 hours. The resulting linker-bound substrate was thoroughly rinsed with pure toluene and methylene chloride, and dried for about 18 hours under reduced pressure.