반응 #1148662
ord-d6f5b387ebd944f0835dcb70f656f92a
반응 조건
후처리
- 1기타to react for at least 24 hours at room temperature
- 2기타To separate fluorescent-Desferrioxamine from free fluorescein and free Desferrioxamine
- 3세척are eluted in 2 ml fractions
- 4세척The fluorescein-Desferrioxamine is eluted in the void volume of the column
- 5기타The first peak is collected
- 6기타used for preparing fluorescein-Desferrioxamine, Desferrioxamine, and rhodamine-liposomes
실험 절차
An aliquot of 120 mg of Desferrioxamine and 233.64 mg of fluorescein isothiocyanate (FITC) are mixed in 50 ml of 0.1 M Na carvonate pH 9.5. A molar ratio of 3:1 of FITC: Desferrioxamine is used as described in Hentz et al. (1997) Anal. Chem. 69:4994-5000. The reaction mixture is wrapped in aluminum foil to avoid light exposure and allowed to react for at least 24 hours at room temperature. To separate fluorescent-Desferrioxamine from free fluorescein and free Desferrioxamine, the mixture is electrophoresed over a P-2 column (Bio-Rad) equilibrated in saline and samples are eluted in 2 ml fractions. The fluorescein-Desferrioxamine is eluted in the void volume of the column. The first peak is collected and used for preparing fluorescein-Desferrioxamine, Desferrioxamine, and rhodamine-liposomes.