반응 #1144396

ord-76464133d3cd419599233faa4ea6c728

반응 방정식

[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-]
sodium tetraborate
O=C([O-])C(=O)Cc1c[nH]c2ccccc12
indole-3-pyruvate
O=[As]([O-])([O-])[O-].[Na+].[Na+].[Na+]
sodium arsenate
O=C([O-])C(=O)Cc1c[nH]c2ccccc12
indole-3-pyruvate
O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O
EDTA
→
N[C@H](Cc1c[nH]c2ccccc12)C(=O)O
D-Tryptophan

반응물

[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-]
sodium tetraborate
O=C([O-])C(=O)Cc1c[nH]c2ccccc12
indole-3-pyruvate
O=[As]([O-])([O-])[O-].[Na+].[Na+].[Na+]
sodium arsenate
O=C([O-])C(=O)Cc1c[nH]c2ccccc12
indole-3-pyruvate
O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O
EDTA

시약

없음

용매

—
following solution

반응 조건

상세 조건
See reaction.notes.procedure_details.

후처리

  1. 1
    기타The extent of reaction
  2. 2
    기타formed
  3. 3
    기타to 5 μl, 10 μl and 20 μl of reaction
  4. 4
    workup.ADDITIONmix
  5. 5
    기타prepared in the same solution

실험 절차

The extent of reaction was followed by measuring the amount of indole-3-pyruvate formed using the following assay: to 5 μl, 10 μl and 20 μl of reaction mix, 200 μl of the following solution was added: 0.5 mM sodium arsenate; 0.5 mM EDTA; and 50 mM sodium tetraborate (pH 8.5). Absorbance of the indole-3-pyruvate enol-borate complex at 325 nm was compared to a standard curve of indole-3-pyruvate prepared in the same solution.

출처

DOI: 10.6084/m9.figshare.5104873.v1특허: US09399763B2uspto-grants-2016_07