반응 #10474

반응 방정식

cis-3-benzyloxycyclohexan-1-ol

→

3-benzyloxycyclohexan-1-ol

용매

vinyl acetate

methylene chloride

반응 조건

후처리

1. 1
   기타After leaving
2. 2
   workup.WAITto stand overnight
3. 3
   여과the enzyme was filtered off
4. 4
   기타A sample was withdrawn
5. 5
   농축concentrated by evaporation under reduced pressure

실험 절차

20.3 g of cis-3-benzyloxycyclohexan-1-ol were dissolved in 35 ml of vinyl acetate and 125 ml of methylene chloride, admixed with 2.0 g of Novozym 435 and stirred at 20–23° C. for 6 h. After leaving to stand overnight, the enzyme was filtered off. A sample was withdrawn and concentrated by evaporation under reduced pressure. The enantiomeric excess of (1S,3R)-3-benzyloxycyclohexan-1-ol was >99% (HPLC on Chiralpak AD-H 250×4.6; 1 ml/min, 25:1:0.5 heptane/EtOH/CH3CN+0.1% TFA), and the enantiomeric excess of the (1R,3S)-acetate was 78% (HPLC on Chiralcel OD 250×4.6; 1 ml/min, 100:1:0.5 heptane/EtOH/CH3CN).

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