반응 #10257
ord-2c4f74d36a3e4147b27538559e142388
반응 방정식
NaCl
EDTA
LTA4
LTA4 methyl ester
LTA4
→
LTB4
반응 조건
상세 조건
See reaction.notes.procedure_details.
실험 절차
The LTA4 substrate was freshly prepared by alkaline hydrolysis of LTA4 methyl ester (Cayman Chemical Co., USA). After incubating for 10 minutes in the presence of LTA4 (1 μM in 50 mM Tris-HCl, 0.15 M NaCl, 0.5% BSA, pH 7.4), the reaction is stopped by diluting 1/20 in 0.1 M potassium phosphate buffer containing 1.5 mM NaN2, 0.4 M NaCl, 1 mM EDTA, 0.1% BSA, pH 7.4, −4° C.