반응 #1004442

반응 방정식

glucose

NaCl

cysteine hydrochloride

calcium carbonate

→

2-hydroxyisocaproic acid

반응 조건

후처리

1. 1
   기타the mixture formed
2. 2
   workup.ADDITIONwas then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes)
3. 3
   workup.ADDITIONThereafter, the culture medium treated

실험 절차

210 parts by weight of this glucose was mixed with 11,600 parts by weight of a culture medium (10 g/l of peptone, 5 g/l of yeast extract, 2 g/l of meat extract, 5 g/l of NaCl, 2 g/l of cysteine hydrochloride and 5 g/l of calcium carbonate), and the mixture formed was injected into a pressure bottle. After the gaseous-phase portion in the bottle was displaced with nitrogen gas, the bottle was hermetically closed with a butyl rubber stopper, which was then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes). Thereafter, the culture medium treated was cooled to 30° C., and 116 parts by weight of a pre-culture fluid of 2-hydroxyisocaproic acid productive strain HICA432 isolated in the manner as described previously was added thereto by means of a syringe when the bottle was kept hermetically stoppered, followed by a static culture at 30° C. for 3 days. Thereafter, the procedure in Example 1 was repeated to collect 9 parts by weight of 2-hydroxyisocaproic acid.

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