反応 #997159

ord-e6bb07a321674d3ab4cfc12904b0685f

反応方程式

NC(CO)(CO)CO.O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate Tris
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
phosphate buffered saline
→
CC(C)=CCC/C(C)=C/CC/C(C)=C/CC/C=C(\C)CC/C=C(\C)CCC=C(C)C
MF59

反応物

—
MF59-0
NC(CO)(CO)CO.O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate Tris
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
phosphate buffered saline
—
2
—
MF59-0

試薬

なし

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    その他to yield a total of 0.7 ml
  2. 2
    workup.ADDITIONThe composition was gently mixed
  3. 3
    その他produced in Example 1
  4. 4
    その他to yield a total of 3 ml
  5. 5
    workup.ADDITIONThe composition was gently mixed

実験手順

10 Baboons were divided into two groups (five baboons per group) and administered the formulations specified in Table 1. For Group 1, equal parts of the adjuvant MF59-0, and p24gag/sf2 (in a citrate/Tris buffer) were combined to yield a total of 0.7 ml. The composition was gently mixed and 500 p1 (to yield 100 μg p24gag/sf2/dose) of vaccine was injected intramuscularly (IM) in the thigh muscle. For Group 2, 1.5 ml of 2× phosphate buffered saline (PBS) was added to 46.8 mg of the PLG-entrapped p24gag/sf2 formulation produced in Example 1. The material was vortexed for about 30 seconds until all beads were in suspension. 1.5 ml of MF59-0 was added to the resuspended beads to yield a total of 3 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected IM in the thigh muscle.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US08021834B2uspto-grants-2011_09