反応 #969876

反応方程式

piperidine

Fmoc-Val-Pro doxorubicin

→

compound G

収率 50.0%

H-Val-Pro doxorubicin

収率 50.0%

溶媒

dimethylformamide

反応条件

後処理

1. 1
   その他form red to purple)
2. 2
   その他Then, the reaction mixture was evaporated to dryness under reduced pressure
3. 3
   その他the residue thus obtained
4. 4
   その他was purified by reverse phase chromatography with water/acetonitrile (70:1)

実験手順

A solution of piperidine in dimethylformamide 50% (1.92 mL) was added to Fmoc-Val-Pro-doxorubicin (F) (29.7 mg, 0.03 mmol) and the reaction mixture was stirred at room temperature for 1 minute (the reaction colour changes form red to purple). Then, the reaction mixture was evaporated to dryness under reduced pressure and the residue thus obtained was purified by reverse phase chromatography with water/acetonitrile (70:1) to give the deprotected compound G (50% yield)

出典