反応 #939015

反応方程式

LTC4

LTA4 methyl ester

serine borate

→

Leukotriene C4

溶媒

phosphate

反応条件

後処理

1. 1
   その他for 30 minutes
2. 2
   その他at 37° C
3. 3
   その他The reaction was terminated by addition of ice-cold methanol

実験手順

LTC4 synthase prepared as a crude fraction from guinea pig lung was used. The test compounds7were tested in duplicate at a concentration of 300 μg/ml. The test compound and/or vehicle was incubated with 12 μg enzyme, 0.3 μg LTA4 methyl ester, 0.2% (w/w) albumin (to stabilize the product) and 4.5 mM serine borate (to prevent conversion of LTC4 to LTD4) in phosphate buffer pH 7.8 for 30 minutes at 37° C. The reaction was terminated by addition of ice-cold methanol. Formation of LTC4 was quantitated by RIA (radioimmunoassay).

出典