反応 #75004

ord-d12b3c586e7f4196ac0a0810b72b08cc

反応方程式

[Na+].[OH-]
NaOH
CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](OC(=O)CCC(=O)O)CC[C@]4(C)[C@H]3CC[C@]12C
cholesteryl hydrogen succinate
CCCCN(CCCC)CCCC
tributylamine
CC(C)COC(=O)Cl
isobutyl chloroformate
→
CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C
cholesterol

反応物

[Na+].[OH-]
NaOH
CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](OC(=O)CCC(=O)O)CC[C@]4(C)[C@H]3CC[C@]12C
cholesteryl hydrogen succinate
CCCCN(CCCC)CCCC
tributylamine
CC(C)COC(=O)Cl
isobutyl chloroformate

試薬

なし

溶媒

C1COCCO1
Dioxane
O
water
C1COCCO1
p-dioxane

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    温度The solution was cooled
  2. 2
    その他cold for 30 minutes
  3. 3
    workup.ADDITIONthe mixture was added in one portion to a stirred
  4. 4
    温度cooling
  5. 5
    その他The material was then dialyzed against water

実験手順

20.25 Grams (41.8 mmoles) of cholesteryl hydrogen succinate (sold by Aldrich Chemicals) and 19.8 ml (83.4 mmoles) of tributylamine were dissolved in 540 ml of Dioxane. The solution was cooled and treated with 5.3 ml (5.7 g, 41.7 mmoles) of isobutyl chloroformate. The reaction was permitted to proceed in the cold for 30 minutes, and then the mixture was added in one portion to a stirred, cooled solution of 50.0 g (41.7 mmoles) of bovine serum albumin in 1100 ml of water, 750 ml of p-dioxane, and 50 ml of 1 N NaOH. Stirring and cooling was continued for 6 hours. The material was then dialyzed against water and lyophilized to yield a stable white powder useful as a cholesterol standard.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US04175074uspto-grants-1979_11